PoplarV1, Main, Exploration, bibRecord, 002A60

Genome-wide identification and expression analysis of heat-responsive and novel microRNAs in Populus tomentosa.

Identifieur interne : 002A60 ( Main/Exploration ); précédent : 002A59; suivant : 002A61

Genome-wide identification and expression analysis of heat-responsive and novel microRNAs in Populus tomentosa.

Auteurs : Lei Chen [République populaire de Chine] ; Yuanyuan Ren ; Yiyun Zhang ; Jichen Xu ; Fengshuo Sun ; Zhiyi Zhang ; Yanwei Wang

Source :

Gene [ 1879-0038 ] ; 2012.

RBID : pubmed:22634103

Descripteurs français

KwdFr :
- Populus (génétique), Réaction de polymérisation en chaine en temps réel (MeSH), Température élevée (MeSH), microARN (génétique).
MESH :
- génétique : Populus, microARN.
- Réaction de polymérisation en chaine en temps réel, Température élevée.

English descriptors

KwdEn :
- Hot Temperature (MeSH), MicroRNAs (genetics), Populus (genetics), Real-Time Polymerase Chain Reaction (MeSH).
MESH :
- chemical , genetics : MicroRNAs.
- genetics : Populus.
- Hot Temperature, Real-Time Polymerase Chain Reaction.

Abstract

Plant microRNAs have a vital role in various abiotic stress responses by regulating gene expression. Heat stress is one of the most severe abiotic stresses, and affects plant growth and development, even leading to death. To identify heat-responsive miRNAs at the genome-wide level in Populus, Solexa sequencing was employed to sequence two libraries from Populus tomentosa, treated and untreated by heat stress. Sequence analysis identified 134 conserved miRNAs belonging to 30 miRNA families, and 16 novel miRNAs belonging to 14 families. Among these miRNAs, 52 miRNAs from 15 families were responsive to heat stress and most of them were down-regulated. qRT-PCR analysis confirmed that the conserved and novel miRNAs were expressed in P. tomentosa, and revealed similar expression trends to the Solexa sequencing results obtained under heat stress. One hundred and nine targets of the novel miRNAs were predicted. This study opens up a new avenue for understanding the regulatory mechanisms of miRNAs involvement in the heat stress response of trees.

DOI: 10.1016/j.gene.2012.05.034
PubMed: 22634103

Affiliations:

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Le document en format XML

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<author><name sortKey="Chen, Lei" sort="Chen, Lei" uniqKey="Chen L" first="Lei" last="Chen">Lei Chen</name>
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<author><name sortKey="Xu, Jichen" sort="Xu, Jichen" uniqKey="Xu J" first="Jichen" last="Xu">Jichen Xu</name>
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<author><name sortKey="Zhang, Zhiyi" sort="Zhang, Zhiyi" uniqKey="Zhang Z" first="Zhiyi" last="Zhang">Zhiyi Zhang</name>
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<author><name sortKey="Wang, Yanwei" sort="Wang, Yanwei" uniqKey="Wang Y" first="Yanwei" last="Wang">Yanwei Wang</name>
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<front><div type="abstract" xml:lang="en">Plant microRNAs have a vital role in various abiotic stress responses by regulating gene expression. Heat stress is one of the most severe abiotic stresses, and affects plant growth and development, even leading to death. To identify heat-responsive miRNAs at the genome-wide level in Populus, Solexa sequencing was employed to sequence two libraries from Populus tomentosa, treated and untreated by heat stress. Sequence analysis identified 134 conserved miRNAs belonging to 30 miRNA families, and 16 novel miRNAs belonging to 14 families. Among these miRNAs, 52 miRNAs from 15 families were responsive to heat stress and most of them were down-regulated. qRT-PCR analysis confirmed that the conserved and novel miRNAs were expressed in P. tomentosa, and revealed similar expression trends to the Solexa sequencing results obtained under heat stress. One hundred and nine targets of the novel miRNAs were predicted. This study opens up a new avenue for understanding the regulatory mechanisms of miRNAs involvement in the heat stress response of trees.</div>
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<CopyrightInformation>Copyright © 2012 Elsevier B.V. All rights reserved.</CopyrightInformation>
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Genome-wide identification and expression analysis of heat-responsive and novel microRNAs in Populus tomentosa.

Genome-wide identification and expression analysis of heat-responsive and novel microRNAs in Populus tomentosa.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki